The aim of the study was to find out the impact of 18-weeks moderate aerobic exercise programme on lipid profile in the middle-aged male and female. The 40 middle-aged men were randomly assigned to exercise (n= 20) and control groups (n= 20) and age ranged from 45 to 40 years. While the exercise group participated in 18 weeks physical exercise program (walking or cycling), the control group maintained their previous exercise habits. Serum triglycerides decreased from 1.50 ± 0.11 to 1.28 ± 0.07 mmol/l (p<0.01) and high-density lipoprotein cholesterol increased from 1.20 ± 0.04 to 1.41 ± 0.03 mmol/l (p<0.01) in the exercise group after 18 weeks exercise intervention. It is concluded that the change in lipid profile (triglycerides) in the exercise group was not dependent on weight reduction but high-density lipoprotein cholesterol changed based on weight reduction.