The purpose of the present investigation was to compare the physiological variables among different distance runners (short distance, middle distance and long distance runners). For this study total thirty (30) male subjects; ten of each distance were randomly selected from Institute of Professional Studies, College of Physical Education, Gwalior (M.P.). The age of all subjects ranged between 20-30 years. The data was collect for each variable administering their respective tests. To ensure that the data was reliable, each subject was given sufficient time to perform the tests for each variable. To analyse the collected data one way analysis of variance (ANOVA) was used for each variable separately for both Physical and Physiological variables. Level of significance was set at 0.05. The study reveals there is significant difference in case of Vital capacity and Heart rate but Systolic and Diastolic blood pressure did not show any significant difference.